Empresa Productos Distribuidores Servicios Genómicos Zona Cliente


Productos ecogen  
 

 
JetSeq™ NGS Library Preparation Kit-Secuenciación
 

Bioline JetSeq DNA Library Preparation Kit provides all of the enzymes and reaction buffers required for constructing fragmented libraries for NGS and includes the following: end repair, A-tailing, ligation, and amplification. •Increased sensitivity for limiting amounts of input sample •Improved yield from fewer steps, reducing risk of sample loss •Faster protocol for earlier results and increased throughput •Reliable library preparation from even very challenging samples •Conveniently formulated, enabling indexing of up to 16 samples.

The success of next-generation sequencing is dependent upon the precise and accurate processing of the input DNA. This requires high quality library preparation from sheared DNA and relies on a coordinated series of standard molecular biology reactions whilst maintaining high yields during the intermediate purification steps.
 
Features & Benefits
  • Low input: end-repair, A-tailing and ligation combined in the same tube, thereby eliminating cleanup steps and improving sample yield
  • Improved confidence: simpler automatable protocol with fewer steps for reducing the risk of sample loss, offering greater peace-of-mind
  • Increased speed: library preparation in less than 3 hours for reduced time-to-results and increased sample throughput
  • Highly efficient: reaction buffer pre-optimized to provide maximum reaction efficiency and highest conversion rates
  • Improved quality: reliable library preparation from even the most challenging samples providing maximum coverage
  • Maximum convenience: sequencing adaptors, enabling indexing of up to 16 samples, already included in the kit

Instrument Compatibility

JetSeq DNA Library Preparation Kit is compatible with the Illumina MiSeq, NextSeq and HiSeq instruments.

Description

The success of next-generation sequencing is partly dependent upon the precise and accurate processing of the input DNA. High-quality library preparation from sheared DNA requires efficient processing during a series of molecular biology reactions and good recovery during the intermediate purification steps. Bioline has developed an optimized, single-tube library construction method (fig. 1) that enables fast and highly efficient adaptor ligated library construction from a range of sample types and inputs.

The combination of end repair and A-tailing followed by direct ligation of the adaptors reduces the hands-on time, making the process faster and easier to use. Optimized end-repair, A-tailing and ligation steps and the reduction in cleanup steps also offer significant improvement in the yield and quality of sequence-ready libraries, reducing the amount of DNA required.

The JetSeq DNA Library Preparation Kit is highly efficient at converting DNA into an adapter-ligated library resulting in higher sequence coverage. The kit contains all of the enzymes and buffers necessary for highly-efficient conversion of the input DNA to a sequencable, adapter-ligated library (fig. 2).

The JetSeq DNA Library Preparation Kit uses a highly efficient polymerase, together with an optimized buffer formulation to ensure uniform amplification of all genomic regions including those that contain highly variable GC content, thereby reducing bias and ensuring even coverage in subsequent sequencing reactions, resulting in exceptional sequence quality scores even 150 bases into the read (fig. 3), giving greater coverage and reducing the possibility of sequencing gaps.

Specifications /Components

ER Buffer 160 µl
ER Enzyme Mix 96 µl
Ligation buffer 48 µl
Adaptor A 80 µl
Adaptor B 80 µl
Ligase 32 µl
PCR Buffer 80 µl
Primer Mix 80 µl
DNA Polymerase 32 µl
PCR Buffer 80 µl
Primer 16 µl
DNA Polymerase 32 µl
Nuclease Free Water 1.8 ml
Index 1 - 16 20 µl

 

Referencia         Descripción                                               Tamaño

BIO-68025        JetSeq™ NGS Library Preparation Kit        16 Reactions

 

En cualqueir caso, para más información consultar con el dp comercial, o enla web del proveedor (ver abajo).

Documentación: consultar manual del producto y brouchure (ver abajo).







 
     Cantidad:

     Archivos Adjuntos :
Manual del Producto JetSeq DNA Library
Brouchure JetSeq DNA Kit Library Preparation Kit



Introduzca el nombre del producto a buscar   
>> Búsqueda avanzada <<    
Ok
Productos más visitados
Ao Absorbance Microplate Reader-Lector Microplacas -
MyGo Mini -qPCR- /Tiempo real para 16 tubos -
MyGo Pro- qPCR Real Time/Tiempo Real para 32 tubos -
Azure C-Systems. Imagen de Geles Único/Táctil -
BIOTAQ - Polimerasa Termoestable -
nº de productos: 0
Ok
Nuestros distribuidores

Ptge. Dos de Maig, 9-11 . 08041 Barcelona
E-mail: ecogen@ecogen.com


San Hermenegildo, 16 Esc. 1, 1º B .28015 Madrid
E-mail: ecogenm@ecogen.com

 

Nota Legal · Política de cookies · Mapaweb
© Copyright 2001-2012
Diseño web Anunzia (Barcelona)